Session 2

Zid et al., (2014) Promoter sequences direct cytoplasmic localization and translation of mRNAs during starvation in yeast.

Key findings:

The authors used novel approach, ribosome profiling, to identify upregulated mRNAs during yeast starvation. Also they have found that those mRNAs have different translation activity directed by promoter sequence.

The author have demonstrated that promoters contain specific STRE and HSE sequences which recruit stress transcription factors to initiate transcription and guide mRNA, through unknown mechanism, to specific location within the cell (P-body, stress granules or cytoplasm)

 

Relevance of the article:

The information from article has application value in yeast engineering. It shows insights to regulatory mechanisms in protein translation during stress condition, which can be exploited for application purposes. Also they have demonstrated the usage of ribosomal profiling method as a high throughput analysis for detection of translating mRNA with subcodon precision. The construction of synthetic chimeric promoter demonstrated possibility to engineer promoter for specific purposes and usage of STRE and HSE sites can be helpful to translate protein despite overall cell translational inhibition during stress.

 

Rating (‘Exceptional – a must read paper’, ‘Very good – worth spending the time’, ‘Good – only if you have time to spare’).

 

Very good paper

Advertisements
This entry was posted in Journal Club Reports. Bookmark the permalink.

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out / Change )

Twitter picture

You are commenting using your Twitter account. Log Out / Change )

Facebook photo

You are commenting using your Facebook account. Log Out / Change )

Google+ photo

You are commenting using your Google+ account. Log Out / Change )

Connecting to %s